Device

Part:BBa_K1350000:Design

Designed by: JieRui Chen   Group: iGEM14_SZU-China   (2014-09-27)

Ccdb E.coli suicide IPTG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 367


Insert non-formatted text here===Design Notes=== We have been thinking of a way, that way we could eliminate E.coli. Now we Ccdb protein-bound T5-lac and make the controls to suit our needs.

Source

"IPTG bomb" kill the E.coli by coding Ccdb toxin protein. The Ccdb come from BBa_K581008(RBS+ccdB+Termintor) , and T5-lac Promoter from BBa_K592008

References